Assisted hatching improves implantation rates on cryopreserved–thawed embryos. A randomized prospective study

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Assisted hatching improves implantation rates on cryopreserved-thawed embryos. A randomized prospective study.

BACKGROUND Focus on the hatching process has so far been in the field of fresh embryos. Cryopreserved-thawed embryos have a lower rate of pregnancy than fresh embryos, which might be due to hardening of the zona pellucida. METHODS During a 2 year period, a prospective randomized study enrolling 253 cryopreserved-thawed cycles was performed on day 2 embryos. Pseudorandomization to assisted hat...

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Laser-assisted hatching increases pregnancy and implantation rates in cryopreserved embryos that were allowed to cleave in vitro after thawing: a prospective randomized study.

BACKGROUND Cryopreservation of embryos may lead to zona hardening that may compromise in vivo hatching and implantation following thawing and transfer. Assisted hatching (AH) has been advocated as a means of assisting the natural hatching process and enhancing implantation. METHODS The aim of this study was to assess in a prospective randomized manner the effect of laser-assisted hatching (LA...

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In Vitro Fertilization Outcomes Following Assisted Hatching of Embryos with Thick Zona Pellucida—A Prospective Randomized Study

Purpose: Impaired hatching is associated with implantation failure following in vitro fertilization (IVF). Thickening or hardening of the zona pellucida (ZP) has been proposed as a factor in this impairment. We examined whether selective assisted hatching (AH) is beneficial with embryos having a thick ZP. Methods: This prospective, randomized controlled study was performed in the IVF unit of an...

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A randomized double-blind controlled study of the efficacy of laser-assisted hatching on implantation and pregnancy rates of frozen-thawed embryo transfer at the cleavage stage.

BACKGROUND Assisted hatching (AH) in fresh embryo transfer (ET) cycles increases the implantation and pregnancy rates, especially in women with a poor prognosis, repeated implantation failures and in older women. Little information exists in the literature regarding the role of AH in frozen-thawed embryo transfer (FET) cycles. METHODS Embryos were cryopreserved at the cleavage stage. On the d...

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Assisted hatching of human embryos: a systematic review and meta-analysis of randomized controlled trials.

BACKGROUND Assisted hatching (AH) is a manipulation of zona pellucida aiming to facilitate embryo implantation. METHODS Systematic review and meta-analysis of medical literature was used to evaluate the effect of AH on assisted reproduction outcomes: clinical pregnancy, live birth, multiple pregnancy and miscarriage. Additional analysis was performed in these subgroups: (i) fresh embryos tran...

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ژورنال

عنوان ژورنال: Human Reproduction

سال: 2004

ISSN: 1460-2350,0268-1161

DOI: 10.1093/humrep/deh434